Evolution of the miR-290–295/miR-371–373 Cluster Family Seed Repertoire
نویسندگان
چکیده
Expression of the mouse miR-290-295 cluster and its miR-371-373 homolog in human is restricted to early embryos, primordial germ cells, the germ line stem cell compartment of the adult testis and to stem cell lines derived from the early embryonic lineages. Sequencing data suggest considerable seed diversification between the seven homologous pre-miRNAs of miR-290-295 but it is not clear if all of the implied miR-290-295 seeds are also conserved in the human miR-371-373 cluster, which consists of only three homologous pre-miRNAs. By employing miRNA target reporters we show that most, if not all, seeds in miR-290-295 are represented in miR-371-373. In the mouse, pre-miR-290, pre-miR-292 and pre-miR-293 express subsets of the miRNA isoforms processed from the single human pre-miR-371. Comparison of the possible miR-290-295/miR-371-373 seed repertoires in placental mammals suggests a model for the evolution of this miRNA cluster family, which would be otherwise difficult to deduce based solely on pre-miRNA sequence comparisons. The conservation of co-expressed seeds that is characteristic of miR-290-295/miR-371-373 should be taken into account in models of the corresponding miRNA-target interaction networks.
منابع مشابه
Mir-290-295 deficiency in mice results in partially penetrant embryonic lethality and germ cell defects.
Mir-290 through mir-295 (mir-290-295) is a mammalian-specific microRNA (miRNA) cluster that, in mice, is expressed specifically in early embryos and embryonic germ cells. Here, we show that mir-290-295 plays important roles in embryonic development as indicated by the partially penetrant lethality of mutant embryos. In addition, we show that in surviving mir-290-295-deficient embryos, female bu...
متن کاملThe Role of mir-290-295 in Murine Embryonic and Germ Cell Development
MicroRNAs, 22nt long RNAs derived from hairpin transcripts, are important regulators of gene expression and have been shown to participate in regulation of every biological process known to date. Mir-290 through mir-295 (mir-290-295) is a mammalian-specific miRNA cluster that, in the mouse, is expressed specifically in early embryos and embryonic germ cells. This thesis examines the in vivo con...
متن کاملmiR-290/371-Mbd2-Myc circuit regulates glycolytic metabolism to promote pluripotency.
Enhanced glycolysis is a main feature of pluripotent stem cells (PSCs) and is proposed to be important for the maintenance and induction of pluripotency. The molecular mechanism underlying enhanced glycolysis in PSCs is not clear. Using Dgcr8-/- mouse embryonic stem cells (ESCs) that lack mature miRNAs, we found that miR-290 cluster of miRNAs stimulates glycolysis by upregulating glycolytic enz...
متن کاملmiR-20a and miR-290, multi-faceted players with a role in tumourigenesis and senescence
Expression of microRNAs changes markedly in tumours and evidence indicates that they are causatively related to tumourigenesis, behaving as tumour suppressor microRNAs or onco microRNAs; in some cases they can behave as both depending on the type of cancer. Some tumour suppressor microRNAs appear to be an integral part of the p53 and Retinoblastoma (RB) network, the main regulatory pathways con...
متن کاملThe miR-290-295 cluster suppresses autophagic cell death of melanoma cells
We compared the expression levels of 307 miRNAs in six different B16F1 melanoma cell lines of differing malignant properties and found that the miR-290-295 cluster showed a strong upregulation in the more malignant B16F1 daughter cell lines. Its overexpression in B16F1 cells had no major effects on cell proliferation, migration or anchorage-independent growth, but conferred resistance to glucos...
متن کامل